In Vitro Mutagenesis Protocols
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Author | Braman, Jeff |
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Publisher | Humana Press |
Year Published | 2002 |
Dimensions | 15.20 x 1.70 x 24.00 cm |
Language | English |
Condition | New |
Number of Pages | 287 |
Hands-on researchers with proven track records describe in stepwise fashion their advanced mutagenesis techniques. The contributors focus on improvements to conventional site-directed mutagenesis, including a chapter on chemical site-directed mutagenesis, PCR-based mutagenesis and the modifications that allow high throughput mutagenesis experiments, and mutagenesis based on gene disruption (both in vitro- and in situ-based). Additional methods are provided for in vitro gene evolution; for gene disruption based on recombination, transposon, and casette mutagenesis; and for facilitating the introduction of multiple mutations. Time-tested and highly practical, the protocols in In Vitro Mutagenesis Protocols, 2nd Edition offer today’s molecular biologists reliable and powerful techniques with which to illuminate the proteome.
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